10 Things to Consider When Buying 96 well cell culture plate

Guide to Selecting a 96 Well Cell Culture Plate

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Cell culture plates are essential tools in laboratory settings, and understanding how to choose the right one is crucial for successful experiments.

Key Considerations for 96 Well Cell Culture Plates

Before proceeding to select a 96 well cell culture plate, it’s important to consider the following factors:

1. Plate Material

Most cell culture plates are made from polystyrene (PS) which often features surface treatments to promote cell adhesion. Optimal attachment conditions can be achieved if the plates are treated appropriately.

2. Well Shape and Size

• Flat-bottom wells are generally preferred for adherent cells.
• U-shaped wells are suitable for suspension cells.
• V-shaped wells may be useful for certain types of biological assays.

3. Surface Treatment

It is essential that the microplate is either TC-treated to facilitate cell attachment or designed as low-binding to minimize protein absorption, depending on the nature of your cells.

4. Well Number

Choosing between different well numbers should be based on your experimental needs. For example, 6 well plates are great for flow cytometry, while 96 well plates are typically utilized for MTT assays.

5. Volume and Depth

The well volume of the plates can vary, with 96 well plates generally offering lower volumes compared to 6 well or 24 well plates, which affects the total number of samples that can be processed.

6. Compatibility with Equipment

Ensure that the selected plates are compatible with your existing laboratory equipment, such as automated pipetting systems, microplate readers, and washers.

7. Handling Options

When handling 96 well plates, proper techniques must be employed to avoid cross-contamination. Lids or seals can be employed to maintain sterility throughout experiments.

8. Cost Considerations

Cost is always a factor in lab supplies. While high-quality plates may be more expensive, the expense can be justified by improved assay reliability and reproducibility.

9. Sterility and Storage

For cell culture applications, maintaining sterility is essential. Use sterilized plates and store them properly to prevent contamination.

10. Manufacturer's Reputation

Opting for products from reputable manufacturers can help ensure the quality and reliability of your experiment.

To conclude, the selection of a 96 well cell culture plate should follow careful consideration of the factors discussed. The right choices will facilitate successful cell culture experiments.

For further assistance, please explore our offerings including 6 well cell culture plates and 24 well cell culture plates.